Introduction
Adeno-associated virus (AAV) is a ssDNA virus with packaging capacity about 4.7kb. Its propagation is helper-dependent and it is typically not known to cause disease in humans. After genetic engineering, recombinant AAV (rAAV) can remain primarily episomal and can persist in non-dividing cells for a long time without damaging the host cell. Besides, as compared to other used viruses, AAV produces the lowest immune response. These characteristics make AAV an attractive vector system for gene transfer.
Serotype Selection
Below is the list of AAV serotypes we provided:
Production
Storage
AAV particles are dissolved in 0.001% Pluronic F68 PBS and it can store at 4 ℃ for short term (2 weeks), or aliquot and store at -80 ℃ for long term. Please avoid repeated freeze/thaw cycles.
Precaution
1. AAV can be handled at BSL-1, but it should be handled at BSL-2 if AAV expressing oncogenes or toxins.
2. Please choose suitable AAV serotype and ensure the transfer plasmid could express transgene before AAV production.
What services are provided?
1. We provide custom service for the design and construction of your own AAV vector.
(Please refer to C6-16 Plasmid Construction Service)
(1) Different kind of promoters for conditional expression of your transgene
(2) Different transgene-expressing cassettes (e.g., cDNA, shRNA, miRNA or CRISPR/Cas)
2. We provide custom AAV packaging service with various AAV serotypes
(1) C6-22-1 AAV Production Test
(2) C6-22-2 Large Scale AAV Production & Purification
3. Several ready-to-use AAV (Various serotypes) are available for your experiments.
(1) AAV carrying EGFP or mCherry gene
(2) AAV carrying firefly luciferase gene
(3) AAV carrying Cre recombinase transgene
(4) AAV carrying CRISPR/Cas systems (split SpCas9s, ABEmax and BEmax)
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