RNAi資料庫
(Knockdown Information)
clone
ID : TRCN0000296739
TRC / RNAi Core knockdown
Clone ID | Clone Name | Vector Name | Sequence | Symbol | Species | TRC Knockdown Efficiency | Cell | Flag* |
---|---|---|---|---|---|---|---|---|
TRCN0000296739 | NM_019001.3-2464s21c1 | pLKO_TRC005 | GTTACTCACAGGTCGTAAATA | XRN1 | human | 81% | A549 | Good |
* Flag 說明:
Good:qPCR data quality is good
Low qPCR Replicates:Data from less than 3 qPCR replicate wells was used for at least one qPCR assay in the analysis (standard deviation cannot be calculated)
High Error:qPCR replicate wells had high standard deviation (>2 fold, ie dCt stdev > 1)
HighENCt:Detection of endogenous control gene failed (no knockdown calculated)
ENoutlier:Detection of endogenous control gene was at least 2 cycles different than other samples in the same experiment. Assumptions of ΔΔCt analysis might not be valid.
備註:Knockdown 效果在不同細胞會有不同程度的差異
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序號 | Sequencing Result | Cell Type | Assay Day | Validated By | Efficiency | Feedback Date | Photo | 狀態 | ||
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