LOCUS pMD.G 6507 bp DNA circular 2-AUG-2018 SOURCE ORGANISM COMMENT http://www.informaxinc.com/ COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|389466049| COMMENT VNTDBDATE|-12403569| COMMENT LSOWNER| COMMENT VNTNAME|pMD.G| COMMENT VNTAUTHORNAME|Demo User| COMMENT VNTOAUTHORNAME|¤ý©É¤HRNAiCORE-N214| COMMENT VNTREPLTYPE|Plasmid COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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(CObjectList) (CObList) 1 COMMENT (CObList #133=(AnlzFontBackColorItem 3836 3945 46923980) COMMENT #134=(AnlzFontBackColorItem 4298 4912 46923980) COMMENT #135=(AnlzFontBackColorItem 3946 4296 40304639) COMMENT #136=(AnlzFontBackColorItem 4297 4297 46923980))) (CObList) 1735357044 COMMENT (CStringList) 544370548 2083664691 (CObList))) FEATURES Location/Qualifiers promoter 1..750 /vntifkey="29" /label=hCMVp CDS 4837..5697 /vntifkey="4" /label=AmpR intron 801..1348 /vntifkey="15" /label=beta-globin\intron CDS 1436..2971 /vntifkey="4" /label=VSV-G polyA_signal 3115..3895 /vntifkey="25" /label=beta-globin\polyA BASE COUNT 1711 a 1515 c 1480 g 1801 t ORIGIN 1 gagcttggcc cattgcatac gttgtatcca tatcataata tgtacattta tattggctca 61 tgtccaacat taccgccatg ttgacattga ttattgacta gttattaata gtaatcaatt 121 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat 181 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt 241 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa 301 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc 361 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct 421 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag 481 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt 541 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac 601 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc 661 agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc 721 catagaagac accgggaccg atccagcctc cggtcgaccg atcctgagaa cttcagggtg 781 agtttgggga cccttgattg ttctttcttt ttcgctattg taaaattcat gttatatgga 841 gggggcaaag ttttcagggt gttgtttaga atgggaagat gtcccttgta tcaccatgga 901 ccctcatgat aattttgttt ctttcacttt ctactctgtt gacaaccatt gtctcctctt 961 attttctttt cattttctgt aactttttcg ttaaacttta gcttgcattt gtaacgaatt 1021 tttaaattca cttttgttta tttgtcagat tgtaagtact ttctctaatc actttttttt 1081 caaggcaatc agggtatatt atattgtact tcagcacagt tttagagaac aattgttata 1141 attaaatgat aaggtagaat atttctgcat ataaattctg gctggcgtgg aaatattctt 1201 attggtagaa acaactacac cctggtcatc atcctgcctt tctctttatg gttacaatga 1261 tatacactgt ttgagatgag gataaaatac tctgagtcca aaccgggccc ctctgctaac 1321 catgttcatg ccttcttctc tttcctacag ctcctgggca acgtgctggt tgttgtgctg 1381 tctcatcatt ttggcaaaga attcctcgac ggatccctcg aggaattctg acactatgaa 1441 gtgccttttg tacttagcct ttttattcat tggggtgaat tgcaagttca ccatagtttt 1501 tccacacaac caaaaaggaa actggaaaaa tgttccttct aattaccatt attgcccgtc 1561 aagctcagat ttaaattggc ataatgactt aataggcaca gccttacaag tcaaaatgcc 1621 caagagtcac aaggctattc aagcagacgg ttggatgtgt catgcttcca aatgggtcac 1681 tacttgtgat ttccgctggt atggaccgaa gtatataaca cattccatcc gatccttcac 1741 tccatctgta gaacaatgca aggaaagcat tgaacaaacg aaacaaggaa cttggctgaa 1801 tccaggcttc cctcctcaaa gttgtggata tgcaactgtg acggatgccg aagcagtgat 1861 tgtccaggtg actcctcacc atgtgctggt tgatgaatac acaggagaat gggttgattc 1921 acagttcatc aacggaaaat gcagcaatta catatgcccc actgtccata actctacaac 1981 ctggcattct gactataagg tcaaagggct atgtgattct aacctcattt ccatggacat 2041 caccttcttc tcagaggacg gagagctatc atccctggga aaggagggca cagggttcag 2101 aagtaactac tttgcttatg aaactggagg caaggcctgc aaaatgcaat actgcaagca 2161 ttggggagtc agactcccat caggtgtctg gttcgagatg gctgataagg atctctttgc 2221 tgcagccaga ttccctgaat gcccagaagg gtcaagtatc tctgctccat ctcagacctc 2281 agtggatgta agtctaattc aggacgttga gaggatcttg gattattccc tctgccaaga 2341 aacctggagc aaaatcagag cgggtcttcc aatctctcca gtggatctca gctatcttgc 2401 tcctaaaaac ccaggaaccg gtcctgcttt caccataatc aatggtaccc taaaatactt 2461 tgagaccaga tacatcagag tcgatattgc tgctccaatc ctctcaagaa tggtcggaat 2521 gatcagtgga actaccacag aaagggaact gtgggatgac tgggcaccat atgaagacgt 2581 ggaaattgga cccaatggag ttctgaggac cagttcagga tataagtttc ctttatacat 2641 gattggacat ggtatgttgg actccgatct tcatcttagc tcaaaggctc aggtgttcga 2701 acatcctcac attcaagacg ctgcttcgca acttcctgat gatgagagtt tattttttgg 2761 tgatactggg ctatccaaaa atccaatcga gcttgtagaa ggttggttca gtagttggaa 2821 aagctctatt gcctcttttt tctttatcat agggttaatc attggactat tcttggttct 2881 ccgagttggt atccatcttt gcattaaatt aaagcacacc aagaaaagac agatttatac 2941 agacatagag atgaaccgac ttggaaagta actcaaatcc tgcacaacag attcttcatg 3001 tttggaccaa atcaacttgt gataccatgc tcaaagaggc ctcaattata tttgagtttt 3061 taatttttat gaaaaaaaaa aaaaaaaacg gaattcctcg agggatccgt cgaggaattc 3121 actcctcagg tgcaggctgc ctatcagaag gtggtggctg gtgtggccaa tgccctggct 3181 cacaaatacc actgagatct ttttccctct gccaaaaatt atggggacat catgaagccc 3241 cttgagcatc tgacttctgg ctaataaagg aaatttattt tcattgcaat agtgtgttgg 3301 aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt aaaacatcag 3361 aatgagtatt tggtttagag tttggcaaca tatgcccata tgctggctgc catgaacaaa 3421 ggttggctat aaagaggtca tcagtatatg aaacagcccc ctgctgtcca ttccttattc 3481 catagaaaag ccttgacttg aggttagatt ttttttatat tttgttttgt gttatttttt 3541 tctttaacat ccctaaaatt ttccttacat gttttactag ccagattttt cctcctctcc 3601 tgactactcc cagtcatagc tgtccctctt ctcttatgga gatccctcga cggatcggcc 3661 gcaattcgta atcatgtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc 3721 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta 3781 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca 3841 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc 3901 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc 3961 tcactcatag gcggccctaa agttatccac agaatcaggg gataacgcag gaaagaacat 4021 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt 4081 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg 4141 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc 4201 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt 4261 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa 4321 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta 4381 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa 4441 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa 4501 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt 4561 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt 4621 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat 4681 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat 4741 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc 4801 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc 4861 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta 4921 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga 4981 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg 5041 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc 5101 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat 5161 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag 5221 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat 5281 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa 5341 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa 5401 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga 5461 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg 5521 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc 5581 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg 5641 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact 5701 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat 5761 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt 5821 gccacctaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc 5881 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag 5941 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg 6001 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca 6061 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa 6121 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg 6181 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta 6241 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcccattc gccattcagg 6301 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg 6361 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga 6421 cgttgtaaaa cgacggccag tgagcgcgcg taatacgact cactataggg cgaattggag 6481 ctccaccgcg gtggcggccg ctctaga //