LOCUS pLKO_TRC025 7482 bp DNA circular 24-DEC-2008 DEFINITION pLKO.1 TRC v1 library vector, with no insert. SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|494326930| COMMENT VNTDBDATE|502558030| COMMENT LSOWNER| COMMENT VNTNAME|pLKO_TRC025| COMMENT VNTAUTHORNAME|Demo User| COMMENT VNTREPLTYPE|Plasmid COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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5855..6219 /vntifkey="21" /label=gag misc_feature 6365..6606 /vntifkey="21" /label=RRE misc_feature 5751..5888 /vntifkey="21" /label=psi promoter 7004..7254 /vntifkey="29" /label=hU6\(-250) misc_feature 529..1128 /vntifkey="21" /label=Puromycin LTR 1803..2038 /vntifkey="19" /label=3'\LTR\SIN misc_RNA 1144..1732 /vntifkey="53" /label=WPRE polyA_signal 2115..2245 /vntifkey="25" /label=SV40\polyA misc_feature 7255..7271 /vntifkey="21" /label=null-T BASE COUNT 1840 a 1879 c 1956 g 1807 t ORIGIN 1 cccgggttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg 61 cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt 121 cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct 181 ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa 241 gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc 301 cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc 361 cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc 421 gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt 481 gaccgaatca ccgacctctc tccccagggg gatccaccgg agcttaccat gaccgagtac 541 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc 601 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag 661 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg 721 tgggtcgcgg acgacggcgc cgccgtggcg gtctggacca cgccggagag cgtcgaagcg 781 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc 841 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc 901 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg 961 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg 1021 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg 1081 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaac gcgttaagtc 1141 gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt 1201 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc 1261 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag 1321 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc 1381 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc 1441 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg 1501 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg 1561 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc 1621 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt 1681 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcgtcgactt 1741 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg 1801 gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt 1861 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc 1921 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg 1981 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta 2041 cgtatagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat 2101 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca 2161 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac 2221 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc 2281 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat 2341 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt 2401 ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca 2461 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc 2521 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc 2581 ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta 2641 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg 2701 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa 2761 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc 2821 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt 2881 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca 2941 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc 3001 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta 3061 acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat 3121 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc 3181 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct 3241 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag 3301 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta 3361 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc 3421 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca 3481 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg 3541 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg 3601 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca 3661 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa 3721 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa 3781 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata 3841 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat 3901 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc 3961 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata 4021 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt 4081 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga 4141 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag 4201 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa 4261 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag 4321 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg 4381 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat 4441 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta 4501 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg 4561 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc 4621 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa 4681 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc 4741 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt 4801 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct 4861 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc 4921 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg 4981 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt 5041 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc 5101 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc 5161 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct 5221 atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc 5281 tgcaagctta atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag 5341 ttagcaacat gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag 5401 gtggtacgat cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac 5461 cactgaattg ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg 5521 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact 5581 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg 5641 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag 5701 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg 5761 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca 5821 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag 5881 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa 5941 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct 6001 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt 6061 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg 6121 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa 6181 aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga 6241 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt 6301 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg 6361 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc 6421 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa 6481 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa 6541 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg 6601 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg 6661 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga 6721 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga 6781 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa 6841 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg 6901 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc 6961 attatcgttt cagacccacc tcccaacccc gaggggaccc agagagggcc tatttcccat 7021 gattccttca tatttgcata tacgatacaa ggctgttaga gagataatta gaattaattt 7081 gactgtaaac acaaagatat tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg 7141 gtagtttgca gttttaaaat tatgttttaa aatggactat catatgctta ccgtaacttg 7201 aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgaggta ccggacagtt 7261 aaccactttt tgaattctag atcttgagac aaatggcagt attcatccac aattttaaaa 7321 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag 7381 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt 7441 acagggacag cagagatcca ctttggcgcc ggctcgaggg gg //