LOCUS pLKO_AS1008\(PeG 10477 bp DNA circular 24-SEP-2013 DEFINITION pLKO.1 TRC v1 library vector, with no insert. SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|670933039| COMMENT VNTDBDATE|673956663| COMMENT LSOWNER| COMMENT VNTNAME|pLKO_AS1008 (PeGFP-I2-Bsd)| COMMENT VNTAUTHORNAME|Demo User| COMMENT VNTREPLTYPE|Plasmid COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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9.60759 1.46528 #201#)) COMMENT (CObjectList))) (CObjectList)) COMMENT #203=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -973 100) COMMENT (CObjectList) (CObjectList)) COMMENT #204=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -1182 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 16 (CObjectList) (CObList) 1 (CObList)) (CObList) 844320380 COMMENT (CStringList) 1210594096 691162673 (CObList))) FEATURES Location/Qualifiers misc_feature 4535..5341 /vntifkey="21" /label=AMP\r rep_origin complement(5467..6082) /vntifkey="33" /label=pUC\Origin promoter 4..508 /vntifkey="29" /label=hPGK\promoter LTR 6430..6842 /vntifkey="19" /label=5'LTR misc_feature 10308..10425 /vntifkey="21" /label=cPPT rep_origin 3734..4164 /vntifkey="33" /label=F1\ORI misc_feature 6997..7361 /vntifkey="21" /label=gag misc_feature 7507..7748 /vntifkey="21" /label=RRE misc_feature 6893..7030 /vntifkey="21" /label=psi promoter 8146..8397 /vntifkey="29" /label=hU6\(-250) misc_marker 8398..10266 /vntifkey="22" /label=1.9\kb\Stuffer LTR 2945..3180 /vntifkey="19" /label=3'\LTR\SIN misc_RNA 2265..2853 /vntifkey="53" /label=WPRE CDS 531..1250 /vntifkey="4" /label=eGFP misc_feature 1259..1855 /vntifkey="21" /label=IRES CDS 1856..2254 /vntifkey="4" /label=Bsd BASE COUNT 2729 a 2488 c 2434 g 2826 t ORIGIN 1 cccgggttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg 61 cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt 121 cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct 181 ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa 241 gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc 301 cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc 361 cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc 421 gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt 481 gaccgaatca ccgacctctc tccccagggg gatccaatac tgtggccagc atggtgagca 541 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa 601 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga 661 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca 721 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact 781 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg 841 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca 901 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt 961 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg 1021 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc 1081 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca 1141 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt 1201 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa aaactgtgtt 1261 aagatccgcc cctctccctc ccccccccct aacgttactg gccgaagccg cttggaataa 1321 ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg 1381 agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc 1441 gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct 1501 tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac 1561 aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc 1621 cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta 1681 ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg 1741 cctcggtaca catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg 1801 aaccacgggg acgtggtttt cctttgaaaa acacgatgat aatatggcca caaccatggc 1861 caagcctttg tctcaagaag aatccaccct cattgaaaga gcaacggcta caatcaacag 1921 catccccatc tctgaagact acagcgtcgc cagcgcagct ctctctagcg acggccgcat 1981 cttcactggt gtcaatgtat atcattttac tgggggacct tgtgcagaac tcgtggtgct 2041 gggcactgct gctgctgcgg cagctggcaa cctgacttgt atcgtcgcga tcggaaatga 2101 gaacaggggc atcttgagcc cctgcggacg gtgccgacag gtgcttctcg atctgcatcc 2161 tgggatcaaa gccatagtga aggacagtga tggacagccg acggcagttg ggattcgtga 2221 attgctgccc tctggttatg tgtgggaggg ctaaatgggt cgacaatcaa cctctggatt 2281 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg 2341 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct 2401 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc 2461 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca 2521 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac 2581 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt 2641 ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct 2701 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc 2761 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga 2821 cgagtcggat ctccctttgg gccgcctccc cgcggatcca cgcgtgactc tagatgtacc 2881 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg 2941 gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg 3001 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact 3061 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg 3121 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag 3181 tacgtatagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat 3241 atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag 3301 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa 3361 actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc 3421 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt 3481 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc 3541 ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct 3601 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc 3661 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc 3721 gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat 3781 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag 3841 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc 3901 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc 3961 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt 4021 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa 4081 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg 4141 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat 4201 taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt 4261 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct 4321 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc 4381 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa 4441 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg 4501 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt 4561 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg 4621 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac 4681 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc 4741 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa 4801 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc 4861 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt 4921 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga 4981 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa 5041 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa 5101 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa 5161 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt 5221 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt 5281 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg 5341 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt 5401 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca 5461 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac 5521 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac 5581 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct 5641 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg 5701 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca 5761 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt 5821 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta 5881 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc 5941 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc 6001 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa 6061 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag 6121 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg 6181 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga 6241 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat 6301 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag 6361 ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga 6421 gctgcaagct taatgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg 6481 agttagcaac atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta 6541 aggtggtacg atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga 6601 accactgaat tgccgcattg cagagatatt gtatttaagt gcctagctcg atacataaac 6661 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca 6721 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg 6781 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc 6841 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca 6901 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc 6961 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta 7021 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa 7081 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc 7141 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc 7201 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg 7261 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc 7321 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga 7381 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca 7441 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg 7501 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg 7561 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac 7621 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc 7681 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg 7741 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt 7801 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga 7861 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa 7921 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt 7981 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta 8041 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca 8101 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccagagaggg cctatttccc 8161 atgattcctt catatttgca tatacgatac aaggctgtta gagagataat tagaattaat 8221 ttgactgtaa acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt 8281 gggtagtttg cagttttaaa attatgtttt aaaatggact atcatatgct taccgtaact 8341 tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgagg taccggtgtg 8401 ttgtaaatga gcacacaaaa tacacatgct aaaatattat attctatgac ctttataaaa 8461 tcaaccaaaa tcttcttttt aataacttta gtatcaataa ttagaatttt tatgttcctt 8521 tttgcaaact tttaataaaa atgagcaaaa taaaaaaacg ctagttttag taactcgcgt 8581 tgttttcttc acctttaata atagctactc caccacttgt tcctaagcgg tcagctcctg 8641 cttcaatcat tttttgagca tcttcaaatg ttctaactcc accagctgct ttaactaaag 8701 cattgtcttt aacaactgac ttcattagtt taacatcttc aaatgttgca cctgattttg 8761 aaaatcctgt tgatgtttta acaaattcta atccagcttc aacagctatt tcacaagctt 8821 tcatgatttc ttcttttgtt aataaacaat tttccataat acatttaaca acatgtgatc 8881 cagctgcttt ttttacagct ttcatgtctt ctaaaactaa ttcataattt ttgtctttta 8941 atgcaccaat atttaatacc atatcaattt ctgttgcacc atctttaatt gcttcagaaa 9001 cttcgaatgc ttttgtagct gttgtgcatg cacctagagg aaaacctaca acatttgtta 9061 ttcctacatt tgtgcctttt aataattctt tacaatagct tgttcaatat gaattaacac 9121 aaactgttgc aaaatcaaat tcaattgctt catcacataa ttgtttaatt tcagctttcg 9181 tagcatcttg ttttaataat gtgtgatcta tatatttgtt tagtttcatt ttttctccta 9241 tatattcatt tttaatttta attctttaat aatttcgtct actttaactt tagcgttttg 9301 aacagattca ccaacaccta taaaataaat ttttagttta ggttcagttc cacttgggcg 9361 aacagcaaat catgacttat cttctaaata aaattttagt aagtcttgtc ctggcatatt 9421 atacattcca tcgatgtagt cttcaacatt aacaacttta agtccagcaa tttgagttaa 9481 gggtgttgct ctcaatgatt tcattaatgg ttcaattttt aatttctttt cttctggttt 9541 aaaattcaag tttaaagtga aagtgtaata tgcacccatt tctttaaata aatcttctaa 9601 atagtctact aatgttttat tttgtttttt ataaaatcaa gcagcctctg ctattaatat 9661 agaagcttgt attccatctt tatctctagc tgagtcatca attacatatc cataactttc 9721 ttcataagca aaaacaaaat ttaatccgtt atcttcttct ttagcaattt ctctacccat 9781 tcatttaaat ccagttaaag tttttacaat attaactcca tatttttcat gagcgattct 9841 atcacccaaa tcacttgtta caaaacttga atatagagcc ggattttttg gaatgctatt 9901 taagcgtttt agatttgata attttcaatc aattaaaatt ggtcctgttt gatttccatc 9961 taatcttaca aaatgaccat catgttttat tgccattcca aatctgtcag catctgggtc 10021 attcataata ataatatctg catcatgttt aataccatat tcaagcggta tttttcatgc 10081 aggatcaaat tctggatttg gatttacaac atttttaaat gtttcatctt caaatgcatg 10141 ctcttcaacc tcaataacgt tatatcctga ttcacgtaat atttttgggg taaatttagt 10201 tcctgttcca ttaactgcgc taaaaataat ttttaaatct tttttagctt cttgctcttt 10261 tttgtagaat tctagatctt gagacaaatg gcagtattca tccacaattt taaaagaaaa 10321 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata 10381 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg 10441 gacagcagag atccactttg gcgccggctc gaggggg //