LOCUS pLAS3w.RFP-C.Ppu 9910 bp DNA circular 16-AUG-2012 SOURCE ORGANISM COMMENT COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|633013984| COMMENT VNTDBDATE|634554946| COMMENT LSOWNER| COMMENT VNTNAME|pLAS3w.RFP-C.Ppuro| COMMENT VNTAUTHORNAME|Demo User| FEATURES Location/Qualifiers CDS 6121..6978 /vntifkey="4" /label=Amp misc_feature 7182..7722 /vntifkey="21" /label=Ori LTR 4726..4970 /vntifkey="19" /label=HIV\3'-LTR LTR 8436..8616 /vntifkey="19" /label=HIV\5'-LTR misc_feature 8727..8771 /vntifkey="21" /label=Psi\sequence promoter 8207..8435 /vntifkey="29" /label=RSV\Promoter misc_feature 5032..5162 /vntifkey="21" /label=SV40\PolyA promoter 6022..6120 /vntifkey="30" /label=bla\promoter promoter 235..594 /vntifkey="29" /label=CMVieEnh misc_feature 595..1941 /vntifkey="21" /label=Beta-Actinp misc_feature 4066..4654 /vntifkey="21" /label=WPRE promoter 235..1941 /vntifkey="29" /label=CAG\promoter promoter 2926..3429 /vntifkey="29" /label=hPGK CDS 3451..4050 /vntifkey="4" /label=PAC misc_feature 2753..2870 /vntifkey="21" /label=cPPT misc_feature 9282..9523 /vntifkey="21" /label=RRE CDS 1980..2675 /vntifkey="4" /label=RFP-C BASE COUNT 2258 a 2614 c 2812 g 2226 t ORIGIN 1 gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc 61 cattcgatta gtgaacggat ctcgacggta tcgatcacga gactagcctc gaggatccac 121 tagtgaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac 181 gggccagaaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttctagtta 241 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac 301 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc 361 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt 421 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac 481 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac 541 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt 601 cgaggtgagc cccacgttct gcttcactct ccccatctcc cccccctccc cacccccaat 661 tttgtattta tttatttttt aattattttg tgcagcgatg ggggcggggg gggggggggc 721 gcgcgccagg cggggcgggg cggggcgagg ggcggggcgg ggcgaggcgg agaggtgcgg 781 cggcagccaa tcagagcggc gcgctccgaa agtttccttt tatggcgagg cggcggcggc 841 ggcggcccta taaaaagcga agcgcgcggc gggcgggagt cgctgcgttg ccttcgcccc 901 gtgccccgct ccgcgccgcc tcgcgccgcc cgccccggct ctgactgacc gcgttactcc 961 cacaggtgag cgggcgggac ggcccttctc ctccgggctg taattagcgc ttggtttaat 1021 gacggctcgt ttcttttctg tggctgcgtg aaagccttaa agggctccgg gagggccctt 1081 tgtgcggggg ggagcggctc ggggggtgcg tgcgtgtgtg tgtgcgtggg gagcgccgcg 1141 tgcggcccgc gctgcccggc ggctgtgagc gctgcgggcg cggcgcgggg ctttgtgcgc 1201 tccgcgtgtg cgcgagggga gcgcggccgg gggcggtgcc ccgcggtgcg ggggggctgc 1261 gaggggaaca aaggctgcgt gcggggtgtg tgcgtggggg ggtgagcagg gggtgtgggc 1321 gcggcggtcg ggctgtaacc cccccctgca accccctccc cgagttgctg agcacggccc 1381 ggcttcgggt gcggggctcc gtacggggcg tggcgcgggg ctcgccgtgc cgggcggggg 1441 gtggcggcag gtgggggtgc cgggcggggc ggggccgcct cgggccgggg agggctcggg 1501 ggaggggcgc ggcggccccc ggagcgccgg cggctgtcga ggcgcggcga gccgcagcca 1561 ttgcctttta tggtaatcgt gcgagagggc gcagggactt cctttgtccc aaatctgtgc 1621 ggagccgaaa tctgggaggc gccgccgcac cccctctagc gggcgcgggg cgaagcggtg 1681 cggcgccggc aggaaggaaa tgggcgggga gggccttcgt gcgtcgccgc gccgccgtcc 1741 ccttctccct ctccagcctc ggggctgtcc gcggggggac ggctgccttc gggggggacg 1801 gggcagggcg gggttcggct tctggcgtgt gaccggcggc tctagagcct ctgctaacca 1861 tgttcatgcc ttcttctttt tcctacagct cctgggcaac gtgctggtta ttgtgctgtc 1921 tcatcatttt ggcaaagaat ttaatacgac tcactatagg gagacccaag ctggctagca 1981 tgagcgagct gatcaaggag aacatgcaca tgaagctgta catggagggc accgtgaaca 2041 accaccactt caagtgcaca tccgagggcg aaggcaagcc ctacgagggc acccagacca 2101 tgaagatcaa ggtggtcgag ggcggccctc tccccttcgc cttcgacatc ctggctacca 2161 gcttcatgta cggcagcaaa gccttcatca accacaccca gggcatcccc gacttcttta 2221 agcagtcctt ccctgagggc ttcacatggg agagaatcac cacatacgaa gacgggggcg 2281 tgctgaccgc tacccaggac accagcttcc agaacggctg catcatctac aacgtcaaga 2341 tcaacggggt gaacttccca tccaacggcc ctgtgatgca gaagaaaaca cgcggctggg 2401 aggccaacac cgagatgctg taccccgctg acggcggcct gagaggccac agccagatgg 2461 ccctgaagct cgtgggcggg ggctacctgc actgctcctt caagaccaca tacagatcca 2521 agaaacccgc taagaacctc aagatgcccg gcttccactt cgtggaccac agactggaaa 2581 gaatcaagga ggccgacaaa gagacctacg tcgagcagca cgagatggct gtggccaagt 2641 actgcgacct ccctagcaaa ctggggcaca gataaacgcg ttaaggtaca tctagagttt 2701 aaaccctgca ggaattctag atcttgagac aaatggcagt attcatccac aattttaaaa 2761 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag 2821 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt 2881 acagggacag cagagatcca ctttggcgcc ggctcgaggg ggcccgggtt gcgccttttc 2941 caaggcagcc ctgggtttgc gcagggacgc ggctgctctg ggcgtggttc cgggaaacgc 3001 agcggcgccg accctgggtc tcgcacattc ttcacgtccg ttcgcagcgt cacccggatc 3061 ttcgccgcta cccttgtggg ccccccggcg acgcttcctg ctccgcccct aagtcgggaa 3121 ggttccttgc ggttcgcggc gtgccggacg tgacaaacgg aagccgcacg tctcactagt 3181 accctcgcag acggacagcg ccagggagca atggcagcgc gccgaccgcg atgggctgtg 3241 gccaatagcg gctgctcagc agggcgcgcc gagagcagcg gccgggaagg ggcggtgcgg 3301 gaggcggggt gtggggcggt agtgtgggcc ctgttcctgc ccgcgcggtg ttccgcattc 3361 tgcaagcctc cggagcgcac gtcggcagtc ggctccctcg ttgaccgaat caccgacctc 3421 tctccccagg gggatccacc ggagcttacc atgaccgagt acaagcccac ggtgcgcctc 3481 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac 3541 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa 3601 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc 3661 gccgccgtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag 3721 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa 3781 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc 3841 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg 3901 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc 3961 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc 4021 tggtgcatga cccgcaagcc cggtgcctga acgcgttaag tcgacaatca acctctggat 4081 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt 4141 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc 4201 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg 4261 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc 4321 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa 4381 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat 4441 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc 4501 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt 4561 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag 4621 acgagtcgga tctccctttg ggccgcctcc ccgcgtcgac ctttaagacc aatgacttac 4681 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt 4741 cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag 4801 atctgagcct gggagctctc tggctaacta gagaacccac tgcttaagcc tcaataaagc 4861 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga 4921 tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt 4981 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt 5041 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca 5101 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc 5161 tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg 5221 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct 5281 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc 5341 caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg 5401 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc 5461 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct 5521 gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac 5581 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc 5641 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt 5701 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg 5761 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac 5821 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta 5881 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat 5941 ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact 6001 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg 6061 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt 6121 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct 6181 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca 6241 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc 6301 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc 6361 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg 6421 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta 6481 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc 6541 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt 6601 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg 6661 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct 6721 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc 6781 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct 6841 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac 6901 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc 6961 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat 7021 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg 7081 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc 7141 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa 7201 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag 7261 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta 7321 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta 7381 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag 7441 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg 7501 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg 7561 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag 7621 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc 7681 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa 7741 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg 7801 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct 7861 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa 7921 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg 7981 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag 8041 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga 8101 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc 8161 gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg 8221 caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag 8281 agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag 8341 gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag 8401 atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct 8461 gagcctggga gctctctggc taactagaga acccactgct taagcctcaa taaagcttgc 8521 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc 8581 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa 8641 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac 8701 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta 8761 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg 8821 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg 8881 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg 8941 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag 9001 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga 9061 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca 9121 gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg 9181 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa 9241 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt 9301 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca 9361 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc 9421 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg 9481 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac 9541 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga 9601 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa 9661 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg 9721 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata 9781 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac 9841 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc 9901 caaccccgag //