LOCUS pLKO_AS3.1.EGFP3 8653 bp DNA circular 14-MAR-2008 SOURCE ORGANISM COMMENT 1. Seqences at nt6685 to 1 to 1502 were confirmed. COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|476026357| COMMENT VNTDBDATE|476790668| COMMENT LSOWNER| COMMENT VNTNAME|pLKO_AS3.1.EGFP3'| COMMENT VNTAUTHORNAME|Demo User| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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#216=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -1159 100) COMMENT (CObjectList) (CObjectList)) COMMENT #217=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -322 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 10 (CObjectList) (CObList) 1 (CObList)) (CObList) 1735357044 COMMENT (CStringList) 544370548 2083664691 (CObList))) FEATURES Location/Qualifiers CDS 4756..5613 /vntifkey="4" /label=Amp misc_feature 5817..6357 /vntifkey="21" /label=Ori LTR 3361..3605 /vntifkey="19" /label=HIV\3'-LTR LTR 7071..7251 /vntifkey="19" /label=HIV\5'-LTR misc_feature 7927..8131 /vntifkey="21" /label=RRE misc_feature 7362..7406 /vntifkey="21" /label=Psi\sequence promoter 6842..7070 /vntifkey="29" /label=RSV\Promoter misc_feature 3667..3797 /vntifkey="21" /label=SV40\PolyA promoter 4657..4755 /vntifkey="30" /label=bla\promoter promoter 136..495 /vntifkey="29" /label=CMVieEnh misc_feature 496..1842 /vntifkey="21" /label=Beta-Actinp CDS 2558..3278 /vntifkey="4" /label=EGFP misc_feature 1961..2557 /vntifkey="21" /label=IRES2 BASE COUNT 2073 a 2185 c 2414 g 1981 t ORIGIN 1 tcgaggatcc actagtgaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc 61 ttcgcgatgt acgggccaga tatacgcgaa ttgcatgaag aatctgctta gggttaggcg 121 ttttgcgctg cttcctagtt attaatagta atcaattacg gggtcattag ttcatagccc 181 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa 241 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac 301 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca 361 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg 421 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt 481 agtcatcgct attaccatgg tcgaggtgag ccccacgttc tgcttcactc tccccatctc 541 ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat 601 gggggcgggg gggggggggg cgcgcgccag gcggggcggg gcggggcgag gggcggggcg 661 gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt 721 ttatggcgag gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag 781 tcgctgcgtt gccttcgccc cgtgccccgc tccgcgccgc ctcgcgccgc ccgccccggc 841 tctgactgac cgcgttactc ccacaggtga gcgggcggga cggcccttct cctccgggct 901 gtaattagcg cttggtttaa tgacggctcg tttcttttct gtggctgcgt gaaagcctta 961 aagggctccg ggagggccct ttgtgcgggg gggagcggct cggggggtgc gtgcgtgtgt 1021 gtgtgcgtgg ggagcgccgc gtgcggcccg cgctgcccgg cggctgtgag cgctgcgggc 1081 gcggcgcggg gctttgtgcg ctccgcgtgt gcgcgagggg agcgcggccg ggggcggtgc 1141 cccgcggtgc gggggggctg cgaggggaac aaaggctgcg tgcggggtgt gtgcgtgggg 1201 gggtgagcag ggggtgtggg cgcggcggtc gggctgtaac ccccccctgc aaccccctcc 1261 ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtacggggc gtggcgcggg 1321 gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg cggggccgcc 1381 tcgggccggg gagggctcgg gggaggggcg cggcggcccc cggagcgccg gcggctgtcg 1441 aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg cgcagggact 1501 tcctttgtcc caaatctgtg cggagccgaa atctgggagg cgccgccgca ccccctctag 1561 cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg agggccttcg 1621 tgcgtcgccg cgccgccgtc cccttctccc tctccagcct cggggctgtc cgcgggggga 1681 cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg 1741 ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc tcctgggcaa 1801 cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa tttaatacga ctcactatag 1861 ggagacccaa gctggctagc ggcgcgccga attcctgcag ggtttaaact agactcgagc 1921 ggccgccact gtgcacgatg atccgagctc ggtaccaagc ttaagatccg cccctctccc 1981 tccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc 2041 tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc 2101 cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt 2161 ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct 2221 gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa 2281 aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt 2341 tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag 2401 gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggta cacatgcttt 2461 acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt 2521 ttcctttgaa aaacacgatg ataatatggc cacaaccatg ggtgagcaag ggcgaggagc 2581 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt 2641 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca 2701 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg 2761 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg 2821 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca 2881 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg 2941 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca 3001 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga 3061 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc 3121 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc 3181 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg 3241 ccgggatcac tctcggcatg gacgagctgt acaagtaaag cggccctaga tgtaccttta 3301 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga 3361 ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct 3421 ctctggttag accagatctg agcctgggag ctctctggct aactagagaa cccactgctt 3481 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 3541 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt 3601 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt 3661 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat 3721 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat 3781 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa 3841 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag 3901 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag 3961 gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt 4021 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc 4081 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca 4141 acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc 4201 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc 4261 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa 4321 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact 4381 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt 4441 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa 4501 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt 4561 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac 4621 aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa 4681 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat 4741 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg 4801 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa 4861 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt 4921 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt 4981 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat 5041 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg 5101 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta 5161 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat 5221 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag 5281 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa 5341 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca 5401 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc 5461 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt 5521 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc 5581 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat 5641 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt 5701 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac 5761 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc 5821 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca 5881 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta 5941 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct 6001 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg 6061 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc 6121 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta 6181 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg 6241 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt 6301 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg 6361 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg 6421 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc 6481 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg 6541 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt 6601 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca 6661 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct 6721 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat 6781 gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct 6841 taatgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac 6901 atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg 6961 atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga accactgaat 7021 tgccgcattg cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct 7081 ggttagacca gatctgagcc tgggagctct ctggctaact agagaaccca ctgcttaagc 7141 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg 7201 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc 7261 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct 7321 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 7381 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 7441 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 7501 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 7561 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 7621 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 7681 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 7741 taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg 7801 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 7861 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 7921 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc 7981 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 8041 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 8101 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 8161 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 8221 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca 8281 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg 8341 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa 8401 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa 8461 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt 8521 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag 8581 gtggagagag agacagagac agatccattc gattagtgaa cggatctcga cggtatcgat 8641 cacgagacta gcc //