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Plasmid preparation
     TRC protocol: DNA preparation V1
Purification of Transfection-Quality DNA

Lentiviral Production
     TRC protocol: Lentivirus production V3
TRC protocol: Lentivirus production V5

Lentivirus titration
     Estimation of lentivirus titer RIU V1
Estimation of lentivirus titer CFU V1
Estimation of lentivirus titer GFP V1

Lentivirus infection
     TRC protocol: Lentivirus infection V3
TRC protocol: Lentivirus infection V4
Lentivirus Concentration Protocol V1
Establishment of inducible cell line through lentivirus vectors V1
Puromycin Kill Curve
Polybrene Toxicity Test

Screening, Validation and Cell line resources
     TRC Assay Development for HTS Guidelines V1
TRC Knockdown Validation with qPCR
TRC Library Transducible Cell Lines, Literature Summary
TRC Library Transducible Cell Lines, Conditions
PooledScreen SequencingProtocol V2
PooledScreen ChipProtocol V1

The TRC shRNA Design Process
     TRC shRNA design algorithm

Plasmid construction
     shRNA construction-I: PCR method V3
shRNA construction-II: annealing method V3
shRNA construction-III: Method of cloning shRNA using selection marker other than puromycin V2
sticky_end PCR V2

Sequencing Primer
     Sequencing Primer V1

Control shRNA Characterization
Characterization of shRNAs from the CTR01 plate

Useful Tools
     plates to columns Application V1

shRNA Inducible Cell System
     LacO_Protocol V1

Replication-Competent Lentivirus (RCL) 偵測方法
RCL assay

Manual for expressing two shRNAs using one vector
Mannual_2_shRNAs

 
 

 
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