Protocols
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Plasmid preparation
TRC protocol：DNA preparation V1
Purification of Transfection-Quality DNA
Lentiviral Production
TRC protocol：Lentivirus production V3
TRC protocol：Lentivirus production V5
Lentivirus titration
Estimation of lentivirus titer RIU V1
Estimation of lentivirus titer CFU V1
Estimation of lentivirus titer GFP V1
Lentivirus infection
TRC protocol: Lentivirus infection V3
TRC protocol: Lentivirus infection V4
Lentivirus Concentration Protocol V1
Establishment of inducible cell line through lentivirus vectors V1
Puromycin Kill Curve
Polybrene Toxicity Test
Screening, Validation and Cell line resources
TRC Assay Development for HTS Guidelines V1
TRC Knockdown Validation with qPCR
TRC Library Transducible Cell Lines, Literature Summary
TRC Library Transducible Cell Lines, Conditions
PooledScreen SequencingProtocol V2
PooledScreen ChipProtocol V1
The TRC shRNA Design Process
TRC shRNA design algorithm
Plasmid construction
shRNA construction-II: annealing method V3
shRNA construction-I: PCR method V3
shRNA construction-III: Method of cloning shRNA using selection marker other than puromycin V2
sticky_end PCR V2
Sequencing Primer
Sequencing Primer V1
Control shRNA Characterization
Characterization of shRNAs from the CTR01 plate
Useful Tools
plates to columns Application V1
shRNA Inducible Cell System
LacO_Protocol V1
TetO_Protocol V1
Replication-Competent Lentivirus (RCL) 偵測方法
RCL assay
Manual for expressing two shRNAs using one vector
Mannual_2_shRNAs
CRISPR 相關
sgRNA cloning Protocol V1
Surrogate cloning Protocol V1
Protocol for gene knock-out by using CRISPR technique
Technique report for miRNA loss-of-function studies by using CRISPR
miRNA Sponge 相關
miRNA sponge protocol V1
Protocol for miRNA loss-of-function study by using miRNA sponges