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| Plasmid preparation |
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TRC protocol: DNA preparation V1
Purification of Transfection-Quality DNA
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| Lentiviral Production |
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TRC protocol: Lentivirus production V3
TRC protocol: Lentivirus production V5
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| Lentivirus titration |
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Estimation of lentivirus titer RIU V1
Estimation of lentivirus titer CFU V1
Estimation of lentivirus titer GFP V1
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| Lentivirus infection |
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TRC protocol: Lentivirus infection V3
TRC protocol: Lentivirus infection V4
Lentivirus Concentration Protocol V1
Establishment of inducible cell line through lentivirus vectors V1
Puromycin Kill Curve
Polybrene Toxicity Test
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| Screening, Validation and Cell line resources |
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TRC Assay Development for HTS Guidelines V1
TRC Knockdown Validation with qPCR
TRC Library Transducible Cell Lines, Literature Summary
TRC Library Transducible Cell Lines, Conditions
PooledScreen SequencingProtocol V2
PooledScreen ChipProtocol V1
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| The TRC shRNA Design Process |
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TRC shRNA design algorithm
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| Plasmid construction |
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shRNA construction-I: PCR method V3
shRNA construction-II: annealing method V3
shRNA construction-III: Method of cloning shRNA using selection marker other than puromycin V2
sticky_end PCR V2
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| Sequencing Primer |
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Sequencing Primer V1
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| Control shRNA Characterization |
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Characterization of shRNAs from the CTR01 plate
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| Useful Tools |
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plates to columns Application V1
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| shRNA Inducible Cell System |
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LacO_Protocol V1
TetO_Protocol V1
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| Replication-Competent Lentivirus (RCL) 偵測方法 |
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RCL assay
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| Manual for expressing two shRNAs using one vector |
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Mannual_2_shRNAs
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| CRISPR 相關 |
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sgRNA cloning Protocol V1
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Surrogate cloning Protocol V1
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Protocol for gene knock-out by using CRISPR technique
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Technique report for miRNA loss-of-function studies by using CRISPR
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| miRNA Sponge 相關 |
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miRNA sponge protocol V1
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Protocol for miRNA loss-of-function study by using miRNA sponges
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